Microscopic investigations of palynological features of convolvulaceous species from arid zone of Pakistan

Microsc Res Tech. 2018 Feb;81(2):228-239. doi: 10.1002/jemt.22971. Epub 2017 Nov 21.

Abstract

The pollen morphology of some species of glamorous family Convolvulaceae from arid zones of Pakistan has been evaluated. The pollen grains were acetolyzed, measured, described qualitatively, and illustrated using Light microscopy and scanning electron microscopy (SEM). The examined species have differences in shape, size, polarity and exine ornamentation, confirming the eurypalynous character. The pollen types varies from psilate to echinate and colpi to tricolporate, tetracolporate and pantocolporate. Exine ornamentation was exposed as perforate, reticulate and echinate. Spine morphology and exine sculpture are vital for the distinction of species. Pollen fertility shows that selected plants species are well-known in the Arid Zone. A taxonomic key is prepared to use these variations in the identification of species. Statistical analysis by using the Software XLSTAT exhibited that some morphological features is the main characters in identification of the taxa. These studied taxa were separated from each other using cluster Analysis. Our results constructed on MVSP ver. 3.22 software analyses that show morphological explanation; thus, the results highlight the importance of pollen morphology in the identification and characterization of species of the family convolvulacae in arid zone.

Keywords: Convolvulacae; Pakistan; arid zone; pollen morphology.

MeSH terms

  • Convolvulaceae / anatomy & histology*
  • Convolvulaceae / classification
  • Desert Climate
  • Microscopy / methods
  • Microscopy, Electron, Scanning / methods
  • Pakistan
  • Pollen / anatomy & histology
  • Pollen / ultrastructure*
  • Species Specificity