A quantum dot-based lateral flow immunoassay for the sensitive detection of human heart fatty acid binding protein (hFABP) in human serum

Mihaela Savin; Carmen-Marinela Mihailescu; Iulia Matei; Dana Stan; Carmen Aura Moldovan; Marian Ion; Ion Baciu

doi:10.1016/j.talanta.2017.10.045

A quantum dot-based lateral flow immunoassay for the sensitive detection of human heart fatty acid binding protein (hFABP) in human serum

Talanta. 2018 Feb 1:178:910-915. doi: 10.1016/j.talanta.2017.10.045. Epub 2017 Oct 23.

Authors

Mihaela Savin¹, Carmen-Marinela Mihailescu², Iulia Matei³, Dana Stan⁴, Carmen Aura Moldovan⁵, Marian Ion⁵, Ion Baciu⁶

Affiliations

¹ Faculty of Chemistry, University of Bucharest, Regina Elisabeta Bd., No.4-12, Bucharest, Romania; Telemedica, Jean Louis Calderonno.28, Bucharest, Romania.
² DDS Diagnostic, Vatra Luminoasa no.45, 031563 Bucharest, Romania. Electronic address: carmen_mihail28@yahoo.com.
³ Ilie Murgulescu" Institute of Physical Chemistry of the Romanian Academy, 202 Splaiul Independentei, Bucharest 060021, Romania; Department of Physical Chemistry, Faculty of Chemistry, University of Bucharest, Bd. Regina Elisabeta 4-12, Bucharest, Romania.
⁴ Telemedica, Jean Louis Calderonno.28, Bucharest, Romania.
⁵ National Institute for R&D in Microtechnologies-IMT Bucharest, 126 A, Erou Iancu Nicolae Street, Romania.
⁶ Faculty of Chemistry, University of Bucharest, Regina Elisabeta Bd., No.4-12, Bucharest, Romania.

PMID: 29136915
DOI: 10.1016/j.talanta.2017.10.045

Abstract

We describe the preparation and validation of a novel lateral flow immunoassay test for the detection of human heart fatty acid binding protein (hFABP). Water-soluble CdTe quantum dots (QDs) were selected as the fluorescent label and were linked covalently to anti-hFABP antibodies. Upon conjugation, the secondary structure of the anti-hFABP was preserved and the fluorescence quantum yield of the CdTe QDs increased. The labelled antibodies were transferred to the immunoassay test strip and the antigen-antibody reaction was successfully performed. This evidenced the preserved antibody activity of QD-labelled anti-hFABP towards hFABP, and provided a rapid means for the quantitation of hFABP in human serum within the range of 0-160ng ∙ ml^-1, with a much lower detection limit of 221pg.∙ ml^-1 compared with other rapid tests based on lateral flow immunoassays. This new immunoassay test has been successfully used for the early detection of acute myocardial infarction.

Keywords: Acute myocardial infarction; Fluorescence detection; H-FABP; Lateral flow immuno assay; Quantum dots.

MeSH terms

Animals
Blood Chemical Analysis / methods*
Cadmium Compounds / chemistry
Calibration
Fatty Acid-Binding Proteins / blood*
Humans
Immunoassay / methods*
Limit of Detection*
Linear Models
Quantum Dots / chemistry*
Tellurium / chemistry

Substances

Cadmium Compounds
Fatty Acid-Binding Proteins
Tellurium
cadmium telluride