LuxR solos/orphans are very widespread among Proteobacteria; however they are surprisingly understudied given that they are likely to play a major role in cell-cell communication in bacteria. Here we describe three simple methodologies/approaches that can be used in order to begin to study this subgroup of quorum sensing-related LuxR receptors.
Keywords: E. coli; LuxI; LuxR orphans; LuxR solos; Quorum sensing; Regulon; Salmonella; SdiA; Transcriptional fusion; Transposon.