Antibodies raised against synthetic peptides from the Arg-Gly-Asp-containing region of the Leishmania surface protein gp63 cross-react with human C3 and interfere with gp63-mediated binding to macrophages

Infect Immun. 1989 Feb;57(2):630-2. doi: 10.1128/iai.57.2.630-632.1989.

Abstract

The Leishmania surface glycoprotein gp63 binds to complement receptor type 3 on the macrophage surface. Antibody raised against a synthetic peptide containing the Arg-Gly-Asp region of the amino acid sequence of gp63 recognizes both gp63 and the alpha-chain of human C3. Monovalent Fab fragments from this antibody block gp63-mediated binding to macrophages.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Antibodies, Protozoan / immunology*
  • Binding Sites, Antibody
  • Binding, Competitive
  • Complement C3 / immunology*
  • Cross Reactions
  • Humans
  • Leishmania mexicana / immunology*
  • Macrophages / immunology*
  • Macrophages / metabolism
  • Membrane Glycoproteins / immunology*
  • Membrane Glycoproteins / metabolism
  • Membrane Glycoproteins / physiology
  • Molecular Sequence Data
  • Peptide Fragments / chemical synthesis
  • Peptide Fragments / immunology*

Substances

  • Antibodies, Protozoan
  • Complement C3
  • Membrane Glycoproteins
  • Peptide Fragments