Abstract
The Leishmania surface glycoprotein gp63 binds to complement receptor type 3 on the macrophage surface. Antibody raised against a synthetic peptide containing the Arg-Gly-Asp region of the amino acid sequence of gp63 recognizes both gp63 and the alpha-chain of human C3. Monovalent Fab fragments from this antibody block gp63-mediated binding to macrophages.
Publication types
-
Research Support, Non-U.S. Gov't
-
Research Support, U.S. Gov't, P.H.S.
MeSH terms
-
Amino Acid Sequence
-
Animals
-
Antibodies, Protozoan / immunology*
-
Binding Sites, Antibody
-
Binding, Competitive
-
Complement C3 / immunology*
-
Cross Reactions
-
Humans
-
Leishmania mexicana / immunology*
-
Macrophages / immunology*
-
Macrophages / metabolism
-
Membrane Glycoproteins / immunology*
-
Membrane Glycoproteins / metabolism
-
Membrane Glycoproteins / physiology
-
Molecular Sequence Data
-
Peptide Fragments / chemical synthesis
-
Peptide Fragments / immunology*
Substances
-
Antibodies, Protozoan
-
Complement C3
-
Membrane Glycoproteins
-
Peptide Fragments