miR-3065-5p regulates mouse odontoblastic differentiation partially through bone morphogenetic protein receptor type II

Biochem Biophys Res Commun. 2018 Jan 1;495(1):493-498. doi: 10.1016/j.bbrc.2017.11.026. Epub 2017 Nov 7.

Abstract

Illumination of the molecular mechanisms regulating odontoblastic differentiation of dental papilla cells is of great significance for proper dentinogenesis and dental pulp regeneration. In this study, we discovered that microRNA (miR)-3065-5p is up-regulated during odontoblastic differentiation. Overexpression of miR-3065-5p promoted odontoblastic differentiation in vitro. Dual luciferase report assay verified that miR-3065-5p could bind to the 3'UTR of bone morphogenetic protein receptor type II (BMPR2), which dramatically increased in the beginning of odontoblastic differentiation but decreased in the terminal differentiation stage. Inhibition of Bmpr2 in the early stage retarded odontoblastic differentiation while knockdown of Bmpr2 in the terminal stage enhanced odontoblastic differentiation, resembling the effect of miR-3065-5p. Taken together, our present study suggests that miR-3065-5p positively regulates odontoblastic differentiation by directly binding to Bmpr2 in the terminal differentiation stage.

Keywords: Bmpr2; Differentiation; Odontoblast; miR-3065-5p.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 3' Untranslated Regions
  • Animals
  • Bone Morphogenetic Protein Receptors, Type II / genetics*
  • Bone Morphogenetic Protein Receptors, Type II / metabolism
  • Cell Differentiation
  • Cell Line
  • Gene Expression Regulation*
  • Mice
  • MicroRNAs / genetics*
  • MicroRNAs / metabolism
  • Odontoblasts / cytology*
  • Odontoblasts / metabolism
  • RNA, Messenger / genetics
  • Up-Regulation

Substances

  • 3' Untranslated Regions
  • MIRN3065 microRNA, mouse
  • MicroRNAs
  • RNA, Messenger
  • Bmpr2 protein, mouse
  • Bone Morphogenetic Protein Receptors, Type II