Dispersive magnetic immunoaffinity extraction. Anatoxin-a determination

Tinh Le; Francesc A Esteve-Turrillas; Sergio Armenta; Miguel de la Guardia; Guillermo Quiñones-Reyes; Antonio Abad-Fuentes; Antonio Abad-Somovilla

doi:10.1016/j.chroma.2017.10.076

Dispersive magnetic immunoaffinity extraction. Anatoxin-a determination

J Chromatogr A. 2017 Dec 22:1529:57-62. doi: 10.1016/j.chroma.2017.10.076. Epub 2017 Nov 1.

Authors

Tinh Le¹, Francesc A Esteve-Turrillas², Sergio Armenta³, Miguel de la Guardia¹, Guillermo Quiñones-Reyes⁴, Antonio Abad-Fuentes⁴, Antonio Abad-Somovilla⁵

Affiliations

¹ Department of Analytical Chemistry, University of Valencia, 50th Dr. Moliner St., 46100 Burjassot, Spain.
² Department of Analytical Chemistry, University of Valencia, 50th Dr. Moliner St., 46100 Burjassot, Spain. Electronic address: francesc.a.esteve@uv.es.
³ Department of Analytical Chemistry, University of Valencia, 50th Dr. Moliner St., 46100 Burjassot, Spain. Electronic address: sergio.armenta@uv.es.
⁴ Institute of Agrochemistry and Food Technology, Consejo Superior de Investigaciones Científicas (IATA-CSIC), 7th Agustín Escardino Av., 46980 Paterna, Spain.
⁵ Department of Organic Chemistry, University of Valencia, 50th Dr. Moliner St., 46100 Burjassot, Spain.

PMID: 29126587
DOI: 10.1016/j.chroma.2017.10.076

Abstract

Specific monoclonal antibodies were coupled with magnetic Sepharose-based beads and used, for the first time. The methodology was applied to preconcentrate anatoxin-a from water and the later determination by ion mobility spectrometry (IMS). Dispersive magnetic immunoaffinity (d-MagIA) extraction methodology provided a limit of detection of 0.02μgL^-1 and a satisfactory precision with a relative standard deviation lower than 15%. Recoveries were evaluated at 0.5, 1.0 and 5.0μgL^-1 anatoxin-a with quantitative values from 91 to 115%. Additionally, isobaric interferences with phenylalanine were completely avoided by the use of the developed d-MagIA extraction coupled to IMS determinations.

Keywords: Anatoxin-a; Antibody; Cyanobacteria; Immunoaffinity; Ion mobility spectrometry; Magnetic.

MeSH terms

Antibodies, Monoclonal / chemistry*
Cyanobacteria Toxins
Environmental Monitoring / methods*
Ion Mobility Spectrometry
Limit of Detection
Magnetics
Sepharose / chemistry*
Tropanes / analysis*
Water / chemistry*
Water Pollutants, Chemical / analysis

Substances

Antibodies, Monoclonal
Cyanobacteria Toxins
Tropanes
Water Pollutants, Chemical
Water
anatoxin a
Sepharose