Thermodynamic Activity-Based Progress Curve Analysis in Enzyme Kinetics

Trends Biotechnol. 2018 Mar;36(3):234-238. doi: 10.1016/j.tibtech.2017.10.004. Epub 2017 Nov 5.

Abstract

Macrokinetic Michaelis-Menten models based on thermodynamic activity provide insights into enzyme kinetics because they separate substrate-enzyme from substrate-solvent interactions. Kinetic parameters are estimated from experimental progress curves of enzyme-catalyzed reactions. Three pitfalls are discussed: deviations between thermodynamic and concentration-based models, product effects on the substrate activity coefficient, and product inhibition.

Keywords: Margules model; Michaelis–Menten kinetics; solvent effects; thermodynamic activity.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Binding Sites
  • Biocatalysis*
  • Enzymes / chemistry*
  • Hydrophobic and Hydrophilic Interactions
  • Kinetics
  • Models, Theoretical
  • Solvents / metabolism
  • Thermodynamics*
  • Water

Substances

  • Enzymes
  • Solvents
  • Water