Insulin-like growth factor-6 (IGFBP-6) stimulates neutrophil oxidative burst, degranulation and chemotaxis

Inflamm Res. 2018 Feb;67(2):107-109. doi: 10.1007/s00011-017-1107-6. Epub 2017 Oct 30.

Abstract

The aim of this study was to understand whether insulin-like growth factor-binding protein-6 (IGFBP-6) has functional effects on neutrophils, in particular when they cross epithelium during inflammation. We found that IGFBP-6 increased ROS production (cytofluorimetry), degranulation of primary and tertiary granules (ELISA) and transmigration through the epithelial monolayer. No priming by IGFBP-6 on neutrophils stimulated by either PMA or fMLP was observed. IGFBP-6 is an agonist of neutrophils' functions, most likely when these cells have been already activated by other stimuli.

Keywords: Chemotaxis; Degranulation; IGFBP-6; Neutrophils; Oxidative burst.

MeSH terms

  • Cell Degranulation / drug effects*
  • Chemotaxis / drug effects*
  • Cytoplasmic Granules / drug effects
  • Humans
  • In Vitro Techniques
  • Insulin-Like Growth Factor Binding Protein 6 / pharmacology*
  • Matrix Metalloproteinase 9 / metabolism
  • Neutrophils / drug effects*
  • Peroxidase / analysis
  • Peroxidase / metabolism
  • Reactive Oxygen Species / metabolism
  • Respiratory Burst / drug effects*
  • Signal Transduction

Substances

  • Insulin-Like Growth Factor Binding Protein 6
  • Reactive Oxygen Species
  • Peroxidase
  • MMP9 protein, human
  • Matrix Metalloproteinase 9