Determination of Hepatotoxicity in iPSC-Derived Hepatocytes by Multiplexed High Content Assays

Methods Mol Biol. 2018:1683:339-354. doi: 10.1007/978-1-4939-7357-6_19.

Abstract

We present here methods for assessing hepatotoxicity by high content imaging and image analysis. The assays focus on the characterization of toxic effects using a variety of phenotypic endpoint readouts. Multi-parametric automated image analysis is used in the protocols to increase assay sensitivity and provide important information about possible in vitro toxicity mechanisms. iPSC-derived hepatocytes were used as a model for the hepatotoxicity assays, but the methods would also be suitable for other liver toxicity cell models. The methods contain detailed step-by-step descriptions of the cell treatment, staining, image acquisition, and image analysis.

Keywords: Hepatocytes; Hepatotoxicity; High content; Imaging; Screening; Stem cell models.

MeSH terms

  • Autophagy / drug effects
  • Cell Survival / drug effects
  • Cells, Cultured
  • Chemical and Drug Induced Liver Injury
  • Data Interpretation, Statistical
  • Hepatocytes / cytology
  • Hepatocytes / drug effects*
  • Hepatocytes / metabolism*
  • High-Throughput Screening Assays*
  • Humans
  • Image Processing, Computer-Assisted
  • Induced Pluripotent Stem Cells / cytology*
  • Membrane Potential, Mitochondrial / drug effects
  • Molecular Imaging / methods
  • Software
  • Toxicity Tests* / methods