Sequencing and comparative analysis of the Amblyomma sculptum mitogenome

Paulo Henrique Costa de Lima; Rafael Mazioli Barcelos; Raphael Contelli Klein; Pedro Marcus Pereira Vidigal; Carlos Emmanuel Montandon; Mary Hellen Fabres-Klein; Jorge Abdala Dergam; Cláudio Mafra

doi:10.1016/j.vetpar.2017.10.007

Sequencing and comparative analysis of the Amblyomma sculptum mitogenome

Vet Parasitol. 2017 Nov 30:247:121-128. doi: 10.1016/j.vetpar.2017.10.007. Epub 2017 Oct 16.

Authors

Paulo Henrique Costa de Lima¹, Rafael Mazioli Barcelos², Raphael Contelli Klein³, Pedro Marcus Pereira Vidigal⁴, Carlos Emmanuel Montandon⁵, Mary Hellen Fabres-Klein⁶, Jorge Abdala Dergam⁷, Cláudio Mafra⁸

Affiliations

¹ Federal University of Viçosa, Biochemistry and Molecular Biology Department, Minas Gerais, Brazil; Federal Institute of Education, Science and Technology of Amazonas, Amazonas, Brazil. Electronic address: paulolima0027@gmail.com.
² Federal University of Viçosa, Biochemistry and Molecular Biology Department, Minas Gerais, Brazil. Electronic address: maziolirb@gmail.com.
³ Federal University of Viçosa, Biochemistry and Molecular Biology Department, Minas Gerais, Brazil; Federal University of West of Bahia, Bahia, Brazil. Electronic address: rcontelli@gmail.com.
⁴ Federal University of Viçosa, Núcleo de Análise de Biomoléculas (NuBioMol), Minas Gerais, Brazil. Electronic address: pedro.vidigal@ufv.br.
⁵ Federal University of Ouro Preto, Center for Research in Biological Sciences, Minas Gerais, Brazil. Electronic address: em.carlos1984@gmail.com.
⁶ Federal University of Viçosa, Biochemistry and Molecular Biology Department, Minas Gerais, Brazil. Electronic address: mhfabres@gmail.com.
⁷ Federal University of Vicosa, Animal Biology Department, Minas Gerais, Brazil. Electronic address: dergam@ufv.br.
⁸ Federal University of Viçosa, Biochemistry and Molecular Biology Department, Minas Gerais, Brazil. Electronic address: mafra@ufv.br.

PMID: 29080758
DOI: 10.1016/j.vetpar.2017.10.007

Abstract

The mitogenome of Amblyomma sculptum was sequenced, providing important information for understanding the evolutionary relationships among species of the A. cajennense complex. The mitochondrial genome has a circular structure with 37 genes, including 13 coding DNA sequences, two ribosomal RNA genes (12S rRNA and 16S rRNA) and 22 tRNA genes. Comparative analysis with the mitogenomes of six reference species of the genus Amblyomma revealed that the ND5 gene, which is related to energy metabolism, and control regions 1 and 2 of the mitogenomes have polymorphisms that can be exploited as molecular markers to differentiate A. sculptum from other tick species in the Amblyomma cajennense complex as well as other Amblyomma species.

Keywords: Amblyomma cajennense complex; Amblyomma sculptum; Comparative genomics; Mitogenome; Next generation sequencing.

MeSH terms

Animals
Genome, Mitochondrial / genetics*
Genomics*
High-Throughput Nucleotide Sequencing
Ixodidae / genetics*
Molecular Sequence Annotation
Sequence Analysis, DNA