A comparison of estimates of doses to radiotherapy patients obtained with the dicentric chromosome analysis and the γ-H2AX assay: Relevance to radiation triage

Appl Radiat Isot. 2018 Jan:131:1-7. doi: 10.1016/j.apradiso.2017.10.031. Epub 2017 Oct 16.

Abstract

The γ-H2AX assay was investigated as an alternative to the time-consuming dicentric chromosome assay (DCA). Radiation doses to 25 radiotherapy patients were estimated in parallel by DCA and the γ-H2AX assay. The γ-H2AX assay yielded doses in line with the calculated equivalent whole body doses in 92% of the patients, whereas the success rate of DCA was only 76%. The result shows that the γ-H2AX assay can be effectively used as a rapid and more precise alternative to DCA.

Keywords: Biodosimetry, γ-H2AX assay; Dicentric chromosomes; Radiation triage.

Publication types

  • Comparative Study
  • Evaluation Study

MeSH terms

  • Adult
  • Aged
  • Case-Control Studies
  • Chromosome Aberrations*
  • Female
  • Histones / blood
  • Histones / genetics*
  • Humans
  • Male
  • Middle Aged
  • Neoplasms / radiotherapy
  • Radiometry / methods*
  • Radiotherapy Dosage*
  • Reproducibility of Results
  • Whole-Body Irradiation
  • Young Adult

Substances

  • H2AX protein, human
  • Histones