The Separation and the Characterization of Long Chain Fatty Acids and Their Derivatives by Reversed Phase High Performance Liquid Chromatography

Phyllis R Brown; Janet M Beebe; Joseph Turcotte

doi:10.1080/10408348908050843

The Separation and the Characterization of Long Chain Fatty Acids and Their Derivatives by Reversed Phase High Performance Liquid Chromatography

Crit Rev Anal Chem. 1989;21(3):193-208. doi: 10.1080/10408348908050843.

Authors

Phyllis R Brown¹, Janet M Beebe², Joseph Turcotte³

Affiliations

¹ a Department of Chemistry , University of Rhode Island , Kingston.
² b Chemical Process Research with Rohm and Haas Co. , Spring House , Pennsylvania.
³ c Department of Medicinal Chemistry , University of Rhode Island , Kingston.

PMID: 29076360
DOI: 10.1080/10408348908050843

Abstract

The potential for the application of chromatography to the analysis of fatty acids was first realized by A. J. James and A. J. P. Martin in 1952¹. These two noted scientists successfully separated the iso- and ante-isomers of short chain free fatty acids by gas liquid chromatography (GLC). Even today, 35 years later, the method of choice for characterization of fatty acids is capillary gas chromatography with a mass spectrometer as a detector. However, high performance liquid chromatography (HPLC) is now becoming competitive in the separation of fatty acids, especially on the preparative scale.