Genome Editing of Erythroid Cell Culture Model Systems

Jinfen J Yik; Merlin Crossley; Kate G R Quinlan

doi:10.1007/978-1-4939-7428-3_15

Genome Editing of Erythroid Cell Culture Model Systems

Methods Mol Biol. 2018:1698:245-257. doi: 10.1007/978-1-4939-7428-3_15.

Authors

Jinfen J Yik¹, Merlin Crossley¹, Kate G R Quinlan²

Affiliations

¹ School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, NSW, 2052, Australia.
² School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, NSW, 2052, Australia. kate.quinlan@unsw.edu.au.

PMID: 29076095
DOI: 10.1007/978-1-4939-7428-3_15

Abstract

Genome editing to introduce specific mutations or to knock out genes in model cell systems has become an efficient platform for research in the fields of molecular biology, genetics, and cell biology. With recent rapid improvements in genome editing techniques, bench-top manipulation of the genome in cell culture has become progressively easier. The application of this knowledge to erythroid cell culture systems now allows the rapid analysis of the downstream effects of virtually any engineered gene disruption or modification in cell systems. Here, we describe a CRISPR/Cas9-based approach to making genomic modifications in erythroid lineage cells which we have successfully used in both murine (MEL) and human (K562) erythroleukaemia immortalized cell lines.

Keywords: CRISPR/Cas9; Erythrocyte; Genome editing; Genome engineering; K562; MEL.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
CRISPR-Cas Systems
Cell Culture Techniques
Cell Line, Tumor
Cells, Cultured
DNA Mutational Analysis
Erythroid Cells / metabolism*
Gene Editing*
Genes, Reporter
Genome*
Genomics* / methods
Humans
Mutation
Plasmids / genetics
RNA, Guide, CRISPR-Cas Systems

Substances

RNA, Guide, CRISPR-Cas Systems