Cannabis through the looking glass: chemo- and enantio-selective separation of phytocannabinoids by enantioselective ultra high performance supercritical fluid chromatography

G Mazzoccanti; O H Ismail; I D'Acquarica; C Villani; C Manzo; M Wilcox; A Cavazzini; F Gasparrini

doi:10.1039/c7cc06999e

Cannabis through the looking glass: chemo- and enantio-selective separation of phytocannabinoids by enantioselective ultra high performance supercritical fluid chromatography

Chem Commun (Camb). 2017 Nov 14;53(91):12262-12265. doi: 10.1039/c7cc06999e.

Authors

G Mazzoccanti¹, O H Ismail, I D'Acquarica, C Villani, C Manzo, M Wilcox, A Cavazzini, F Gasparrini

Affiliation

¹ Dipartimento di Chimica e Tecnologie del Farmaco, Sapienza Università di Roma, p.le A. Moro 5, 00185 Roma, Italy. francesco.gasparrini@uniroma1.it.

PMID: 29072720
DOI: 10.1039/c7cc06999e

Abstract

By using the Inverted Chirality Columns Approach (ICCA) we have developed an enantioselective UHPSFC method to determine the enantiomeric excess (ee) of (-)-Δ⁹-THC in medicinal marijuana (Bedrocan®). The ee was high (99.73%), but the concentration of the (+)-enantiomer (0.13₅%) was not negligible, and it is worth a systematic evaluation of bioactivity.