Cell tensile technique is an important and widely used tool in cell mechanical research. However, the strain control condition in traditional tensile experiments is not satisfied and would result in big errors. These strain errors will seriously impact the experimental accuracy and decrease the reliability and comparability of experimental results. In order to achieve the accurate strain control of the membrane during stretching, a strain feedback compensation method based on the digital image correlation is proposed in this paper. To evaluate the effect of the proposed compensation method, a series of stretching experiments in different strains ranging from 5% to 20% were performed. The results showed that our proposed method significantly decreased the errors of strain control. These results indicate that the strain feedback compensation method is very effective in controlling strain and can greatly improve the experimental accuracy.