Behavior is one of the most important aspects of animal life. This behavior depends on the link between animals, their nervous systems and their environment. In order to study the behavior of laboratory animals several tools are needed, but a tracking tool is essential to perform a thorough behavioral study. Currently, several visual tracking tools are available. However, they have some drawbacks. For instance, when an animal is inside a cave, or is close to other animals, the tracking cameras cannot always detect the location or movement of this animal. This paper presents RFID Assisted Tracking Tile (RATT), a tracking system based on passive Radio Frequency Identification (RFID) technology in high frequency band according to ISO/IEC 15693. The RATT system is composed of electronic tiles that have nine active RFID antennas attached; in addition, it contains several overlapping passive coils to improve the magnetic field characteristics. Using several tiles, a large surface can be built on which the animals can move, allowing identification and tracking of their movements. This system, that could also be combined with a visual tracking system, paves the way for complete behavioral studies.