Optical microscopy enables the observation of highly magnified objects and material structures on microsurfaces, but it can only acquire 2D images. In order to observe areal features more accurately and intuitively, 3D surface microtopography recovery has been applied to form a 3D surface model of an object from its 2D image sequence. In the 3D reconstruction of the focus evaluation operator, we have the gray variance operator, the gray-scale difference absolute sum operator, the Roberts gradient operator, the Tenengrad gradient operator, the improved Laplace operator, etc. There are two problems with these operators: one is that there is no difference between (x,y) and the gray scale of the pixel in the diagonal direction in the field and the other is that the window size of the focus evaluation operator is fixed, e.g., 3×3, 5×5, etc. Thus, the size of the window for each pixel in the image is the same, and the small window may not cover enough field information while being vulnerable to noise. Large windows can cover more information, but they may result in a smoothing phenomenon, which affects the accuracy of the model. Different pixels around the field have different pixel colors when the size of the window is not the same. Therefore, this paper proposes a modified omnidirectional Laplacian operator with an adaptive window to automatically adjust the size of the window according to the color difference within the window. This also takes into consideration the pixels in the diagonal direction. In addition, very comprehensive verification experiments proved the conclusions.