Temperature-responsive split aptamers coupled with polymerase chain reaction for label-free and sensitive detection of cancer cells

Chem Commun (Camb). 2017 Oct 31;53(87):11889-11892. doi: 10.1039/c7cc06218d.

Abstract

A label-free and general thermo-controlled split apta-PCR strategy was first developed for the sensitive and specific detection of cancer cells. By integrating the temperature-responsive function of split aptamers with PCR amplification, a facile fluorescence assay of liver cancer SMMC-7721 cells was successfully realized with the detection of as low as 100 cells.

MeSH terms

  • Aptamers, Nucleotide / chemistry*
  • Aptamers, Nucleotide / metabolism
  • Biosensing Techniques / methods*
  • Cell Line, Tumor
  • Hep G2 Cells
  • Humans
  • Liver Neoplasms / diagnosis*
  • Liver Neoplasms / metabolism
  • Nucleic Acid Conformation
  • Polymerase Chain Reaction / methods
  • Spectrometry, Fluorescence / methods
  • Temperature

Substances

  • Aptamers, Nucleotide