The detection of hepatitis c virus core antigen using afm chips with immobolized aptamers

T O Pleshakova; A L Kaysheva; J М Bayzyanova; А S Anashkina; V F Uchaikin; V S Ziborov; V A Konev; A I Archakov; Y D Ivanov

doi:10.1016/j.jviromet.2017.10.015

The detection of hepatitis c virus core antigen using afm chips with immobolized aptamers

J Virol Methods. 2018 Jan:251:99-105. doi: 10.1016/j.jviromet.2017.10.015. Epub 2017 Oct 16.

Authors

T O Pleshakova¹, A L Kaysheva², J М Bayzyanova¹, А S Anashkina¹, V F Uchaikin¹, V S Ziborov³, V A Konev⁴, A I Archakov¹, Y D Ivanov¹

Affiliations

¹ Institute of Biomedical Chemistry, Pogodinskaya St. 10, Moscow, 119121, Russia.
² Institute of Biomedical Chemistry, Pogodinskaya St. 10, Moscow, 119121, Russia. Electronic address: kaysheva1@gmail.com.
³ Joint Institute for High Temperatures RAS, Izhorskaya St. 13/19, Moscow, 125412, Russia.
⁴ Pirogov Russian National Research Medical University (RNRMU), Ostrovitianov str. 1, Moscow, 117997, Russia.

PMID: 29042217
DOI: 10.1016/j.jviromet.2017.10.015

Abstract

In the present study, the possibility of hepatitis C virus core antigen (HCVcoreAg) detection in buffer solution, using atomic force microscope chip (AFM-chip) with immobilized aptamers, has been demonstrated. The target protein was detected in 1mL of solution at concentrations from 10^-10М to 10^-13М. The registration of aptamer/antigen complexes on the chip surface was carried out by atomic force microscopy (AFM). The further mass-spectrometric (MS) identification of AFM-registered objects on the chip surface allowed reliable identification of HCVcoreAg target protein in the complexes. Aptamers, which were designed for therapeutic purposes, have been shown to be effective in HCVcoreAg detection as probe molecules.

Keywords: Aptamer; Atomic force microscopy; Core antigen; Mass-spectrometry.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Aptamers, Nucleotide / metabolism*
Microscopy, Atomic Force / methods*
Viral Core Proteins / analysis*

Substances

Aptamers, Nucleotide
Viral Core Proteins
nucleocapsid protein, Hepatitis C virus