Identification of monoclonal antibodies cross-reactive with bottlenose dolphin orthologues of the major histocompatibility complex and leukocyte differentiation molecules

Mahmoud M Elnaggar; Gaber S Abdellrazeq; Stephanie K Venn-Watson; Eric D Jensen; Victoria Hulubei; Lindsay M Fry; Randy E Sacco; William C Davis

doi:10.1016/j.vetimm.2017.09.007

Identification of monoclonal antibodies cross-reactive with bottlenose dolphin orthologues of the major histocompatibility complex and leukocyte differentiation molecules

Vet Immunol Immunopathol. 2017 Oct:192:54-59. doi: 10.1016/j.vetimm.2017.09.007. Epub 2017 Oct 3.

Authors

Mahmoud M Elnaggar¹, Gaber S Abdellrazeq², Stephanie K Venn-Watson³, Eric D Jensen⁴, Victoria Hulubei⁵, Lindsay M Fry⁶, Randy E Sacco⁷, William C Davis⁵

Affiliations

¹ Department of Veterinary Microbiology and Pathology, College of Veterinary Medicine, Washington State University, WA, USA; Department of Microbiology, Faculty of Veterinary Medicine, Alexandria University, Egypt. Electronic address: mahmoud.elnaggar@vetmed.wsu.edu.
² Department of Veterinary Microbiology and Pathology, College of Veterinary Medicine, Washington State University, WA, USA; Department of Microbiology, Faculty of Veterinary Medicine, Alexandria University, Egypt.
³ Translational Medicine and Research Program, National Marine Mammal Foundation, San Diego, CA, USA.
⁴ U.S Navy Marine Mammal Program, Biosciences Division, Space and Naval Warfare Systems Center Pacific, San Diego, CA, USA.
⁵ Department of Veterinary Microbiology and Pathology, College of Veterinary Medicine, Washington State University, WA, USA.
⁶ Animal Disease Research Unit, U.S. Department of Agriculture, Agricultural Research Service, Pullman, WA, 99164, USA.
⁷ Ruminant Diseases and Immunology Research Unit, National Animal Disease Center, Agricultural Research Service, Ames, IA, USA.

PMID: 29042015
DOI: 10.1016/j.vetimm.2017.09.007

Abstract

The slow progress in understanding immunotoxic effects of environmental contaminants and their influence on disease susceptibility in whales is largely due to the limited information available on the immune systems and immune function of species included in the Cetancodontamorpha clade. Studies in species in the other major clades included in the Artiodactylamorpha, Ruminantiamorpha, Suinamorpha, and Camelidamorpha have revealed the immune systems are similar, but not identical. The present study was undertaken to expand the available monoclonal antibody reagents needed to gain insight into the composition, function, and evolution of the immune system in Cetancodontamorpha, using the dolphin (Tursiops truncatus) as a model cetacean species. Screening of a set of mAbs that recognize highly conserved epitopes expressed on the major histocompatibility complex (MHC) and leukocyte differentiation molecules (LDMs) in cattle by flow cytometry revealed some of the mAbs recognize epitopes conserved on dolphin orthologues of MHC class I, MHC class II, CD11a, CD14, CD16, CD18, CD163 and CD172a. Comparison of the amino acid sequences of dolphin and bovine orthologues revealed limited changes in sequence have occurred during speciation, suggesting an approach for developing cross-reactive mAbs for use in cetacean research.

Keywords: Bottlenose dolphins; Flow cytometry; Leukocyte differentiation molecules; Major histocompatibility complex; Monoclonal antibody.

MeSH terms

Animals
Antibodies, Monoclonal / immunology
Antigens, CD / immunology*
Bottle-Nosed Dolphin / immunology
Cross Reactions / immunology*
Female
Flow Cytometry / veterinary
Leukocytes / immunology
Major Histocompatibility Complex / immunology*
Male

Substances

Antibodies, Monoclonal
Antigens, CD