Abstract
From the cultured endophytic fungus Glomerella cingulata isolated from a toxic plant, Gelsemium elegans, one new phenanthrene (1), four new sesquiterpenes (2-5), and three known sesquiterpenes (6-8) were isolated. Their structures were elucidated using spectroscopic methods. Based on the ECD calculations, the absolute configurations of the new compounds were determined. Compounds 2, 4, and 5 inhibited lipopolysaccharide (LPS)-induced NO production in BV2 cells by 50.6, 36.1, and 29.4%, respectively, at 1 μM (positive control curcumin, IC50 = 4.0 μM).
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Animals
-
Curcumin / pharmacology
-
Drugs, Chinese Herbal
-
Endophytes / chemistry*
-
Gelsemium / microbiology
-
Inhibitory Concentration 50
-
Lipopolysaccharides / pharmacology
-
Macrophages / drug effects
-
Mice
-
Molecular Structure
-
Nitric Oxide / biosynthesis
-
Nuclear Magnetic Resonance, Biomolecular
-
Phyllachorales / chemistry*
-
Plants, Toxic
-
Sesquiterpenes / chemistry
-
Sesquiterpenes / isolation & purification*
Substances
-
Drugs, Chinese Herbal
-
Lipopolysaccharides
-
Sesquiterpenes
-
Nitric Oxide
-
Curcumin