Gelatin-Polyaniline Composite Nanofibers Enhanced Excitation-Contraction Coupling System Maturation in Myotubes

Serge Ostrovidov; Majid Ebrahimi; Hojae Bae; Hung Kim Nguyen; Sahar Salehi; Sang Bok Kim; Akichika Kumatani; Tomokazu Matsue; Xuetao Shi; Ken Nakajima; Shizu Hidema; Makoto Osanai; Ali Khademhosseini

doi:10.1021/acsami.7b03979

Gelatin-Polyaniline Composite Nanofibers Enhanced Excitation-Contraction Coupling System Maturation in Myotubes

ACS Appl Mater Interfaces. 2017 Dec 13;9(49):42444-42458. doi: 10.1021/acsami.7b03979. Epub 2017 Dec 4.

Authors

Serge Ostrovidov^{1

2}, Majid Ebrahimi¹, Hojae Bae³, Hung Kim Nguyen¹, Sahar Salehi^{1

4}, Sang Bok Kim⁵, Akichika Kumatani^{1

6}, Tomokazu Matsue^{1

6}, Xuetao Shi⁷, Ken Nakajima⁸, Shizu Hidema⁹, Makoto Osanai^{10

11}, Ali Khademhosseini^{1

2

3

12

13

14

15}

Affiliations

¹ WPI-Advanced Institute for Materials Research, Tohoku University , Sendai 980-8577, Japan.
² Department of Medicine, Center for Biomedical Engineering, Brigham and Women's Hospital, Harvard Medical School , Cambridge, Massachusetts 02139, United States.
³ KU Convergence Science and Technology Institute, Department of Stem Cell and Regenerative Biotechnology, Konkuk University , Hwayang-dong, Kwangjin-gu, Seoul 05029, Republic of Korea.
⁴ Lehrstuhl Biomaterialien, Fakultät für Ingenieurwissenschaften, Universität Bayreuth , Bayreuth 95440, Germany.
⁵ Department of Eco-Machinery system, Korea Institute of Machinery and Materials , Daejeon 305-343, Republic of Korea.
⁶ Graduate School of Environmental Studies, Tohoku University , Sendai 980-8579, Japan.
⁷ National Engineering Research Centre for Tissue Restoration and Reconstruction, South China University of Technology , Guangzhou 510006, PR China.
⁸ School of Materials and Chemical Technology, Tokyo Institute of Technology , Tokyo 152-8550, Japan.
⁹ Graduate School of Agricultural Science, Department of Molecular and Cell Biology, Tohoku University , Sendai 981-8555, Japan.
¹⁰ Department of Radiological Imaging and Informatics, Tohoku University Graduate School of Medicine , Sendai 980-8575, Japan.
¹¹ Department of Intelligent Biomedical Systems Engineering, Graduate School of Biomedical Engineering, Tohoku University , Sendai 980-8575, Japan.
¹² Harvard-MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology , Cambridge, Massachusetts 02139, United States.
¹³ Wyss Institute for Biologically Inspired Engineering, Harvard University , Boston, Massachusetts 02115, United States.
¹⁴ Department of Physics, Faculty of Science, King Abdulaziz University , Jeddah 21569, Saudi Arabia.
¹⁵ California NanoSystems Institute (CNSI), and Center for Minimally Invasive Therapeutics (C-MIT), Department of Bioengineering and Department of Radiology, University of California , Los Angeles, California 90095, United States.

PMID: 29023089
DOI: 10.1021/acsami.7b03979

Abstract

In this study, composite gelatin-polyaniline (PANI) nanofibers doped with camphorsulfonic acid (CSA) were fabricated by electrospinning and used as substrates to culture C2C12 myoblast cells. We observed enhanced myotube formation on composite gelatin-PANI nanofibers compared to gelatin nanofibers, concomitantly with enhanced myotube maturation. Thus, in myotubes, intracellular organization, colocalization of the dihydropyridine receptor (DHPR) and ryanodine receptor (RyR), expression of genes correlated to the excitation-contraction (E-C) coupling apparatus, calcium transients, and myotube contractibility were increased. Such composite material scaffolds combining topographical and electrically conductive cues may be useful to direct skeletal muscle cell organization and to improve cellular maturation, functionality, and tissue formation.

Keywords: C2C12; composite nanofibers; excitation−contraction coupling; gelatin; intracellular calcium; myotube maturation; polyaniline.

MeSH terms

Aniline Compounds
Calcium
Gelatin
Muscle Fibers, Skeletal
Nanofibers*

Substances

Aniline Compounds
polyaniline
Gelatin
Calcium