Carborane-linked 2'-deoxyuridine 5'-O-triphosphate as building block for polymerase synthesis of carborane-modified DNA

Bioorg Med Chem Lett. 2017 Nov 1;27(21):4786-4788. doi: 10.1016/j.bmcl.2017.09.064. Epub 2017 Sep 30.

Abstract

5-[(p-Carborane-2-yl)ethynyl]-2'-deoxyuridine 5'-O-triphosphate was synthesized and used as a good substrate in enzymatic construction of carborane-modified DNA or oligonucleotides containing up to 21 carborane moieties in primer extension reactions by DNA polymerases.

Keywords: Carboranes; DNA polymerase; Nucleoside triphosphates; Nucleotides; Oligonucleotides.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Boranes / chemistry*
  • DNA / biosynthesis*
  • DNA / chemistry
  • DNA Primers / chemistry
  • DNA Primers / metabolism*
  • DNA-Directed DNA Polymerase / metabolism*
  • Deoxyuracil Nucleotides / chemistry*
  • Oligonucleotides / biosynthesis
  • Oligonucleotides / chemistry
  • Polymerase Chain Reaction

Substances

  • Boranes
  • DNA Primers
  • Deoxyuracil Nucleotides
  • Oligonucleotides
  • deoxyuridine triphosphate
  • DNA
  • DNA-Directed DNA Polymerase