Emergence of Various NDM-Type-Metallo-β-Lactamase-Producing Escherichia coli Clinical Isolates in Nepal

Antimicrob Agents Chemother. 2017 Nov 22;61(12):e01425-17. doi: 10.1128/AAC.01425-17. Print 2017 Dec.

Abstract

Of 250 clinical isolates of Escherichia coli obtained in Nepal, 38 were carbapenem resistant, with MICs of imipenem or meropenem of ≥4 μg/ml. All 38 isolates harbored the following blaNDMs: blaNDM-1, blaNDM-3, blaNDM-4, blaNDM-5, blaNDM-7, blaNDM-12, and blaNDM-13 Most of these isolates also harbored the 16S rRNA methylase gene(s) armA, rmtB, and/or rmtC.

Keywords: NDM-type metallo-β-lactamase; carbapenem-resistant Escherichia coli; molecular epidemiology.

MeSH terms

  • Anti-Bacterial Agents / pharmacology*
  • Escherichia coli / drug effects
  • Escherichia coli / enzymology
  • Escherichia coli / genetics*
  • Escherichia coli / isolation & purification
  • Escherichia coli Infections / drug therapy
  • Escherichia coli Infections / epidemiology*
  • Escherichia coli Infections / microbiology
  • Escherichia coli Proteins / genetics
  • Escherichia coli Proteins / metabolism
  • Gene Expression
  • Humans
  • Imipenem / pharmacology
  • Isoenzymes / genetics
  • Isoenzymes / metabolism
  • Meropenem
  • Methyltransferases / genetics
  • Methyltransferases / metabolism
  • Microbial Sensitivity Tests
  • Molecular Epidemiology
  • Multilocus Sequence Typing
  • Mutation*
  • Nepal / epidemiology
  • Plasmids / chemistry
  • Plasmids / metabolism
  • Sequence Analysis, DNA
  • Thienamycins / pharmacology
  • beta-Lactam Resistance / genetics*
  • beta-Lactamases / genetics*
  • beta-Lactamases / metabolism

Substances

  • Anti-Bacterial Agents
  • Escherichia coli Proteins
  • Isoenzymes
  • Thienamycins
  • Imipenem
  • ArmA protein, E coli
  • Methyltransferases
  • RmtB protein, E coli
  • beta-Lactamases
  • beta-lactamase NDM-1
  • Meropenem