Display of DNA on Nanoparticles for Targeting Antigen Presenting Cells

Nicholas M Molino; Medea Neek; Jo Anne Tucker; Edward L Nelson; Szu-Wen Wang

doi:10.1021/acsbiomaterials.7b00148

Display of DNA on Nanoparticles for Targeting Antigen Presenting Cells

ACS Biomater Sci Eng. 2017 Apr 10;3(4):496-501. doi: 10.1021/acsbiomaterials.7b00148. Epub 2017 Mar 14.

Authors

Nicholas M Molino¹, Medea Neek¹, Jo Anne Tucker², Edward L Nelson^{2

3

4}, Szu-Wen Wang^{1

3

5}

Affiliations

¹ Department of Chemical Engineering and Materials Science, University of California, Irvine, CA 92697 USA.
² Department of Medicine, University of California, Irvine, CA 92697 USA.
³ Chao Family Comprehensive Cancer Center, University of California, Irvine, CA 92697 USA.
⁴ Institute for Immunology, University of California, Irvine, CA 92697 USA.
⁵ Department of Biomedical Engineering, University of California, Irvine, CA 92697 USA.

Abstract

Efficient delivery of antigens is of paramount concern in immunotherapies. We aimed to target antigen presenting cells (APCs) by conjugating CpG oligonucleotides to an E2 protein nanoparticle surface (CpG-PEG-E2). Compared to E2 alone, we observed ~4-fold increase of in vitro APC uptake of both CpG-PEG-E2 and E2 conjugated to non-CpG DNA. Furthermore, compared to E2-alone or E2 functionalized solely with polyethylene glycol (PEG), the CpG-PEG-E2 showed enhanced lymph node retention up to at least 48 hr and 2-fold increase in APC uptake in vivo, parameters which are advantageous for vaccine success. This suggests that enhanced APC uptake of nanoparticles mediated by oligonucleotide display may help overcome delivery barriers in vaccine development.

Keywords: CpG; E2; biodistribution; dendritic cell targeting; nanoparticle vaccine; oligonucleotide; polyethylene glycol.

Abstract

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