Cellular and Subcellular Level Localization of Maize Lipids and Metabolites Using High-Spatial Resolution MALDI Mass Spectrometry Imaging

Maria Emilia Dueñas; Adam D Feenstra; Andrew R Korte; Paige Hinners; Young Jin Lee

doi:10.1007/978-1-4939-7315-6_13

Cellular and Subcellular Level Localization of Maize Lipids and Metabolites Using High-Spatial Resolution MALDI Mass Spectrometry Imaging

Methods Mol Biol. 2018:1676:217-231. doi: 10.1007/978-1-4939-7315-6_13.

Authors

Maria Emilia Dueñas^{1

2}, Adam D Feenstra^{1

2}, Andrew R Korte^{1

2}, Paige Hinners¹, Young Jin Lee^{3

4}

Affiliations

¹ Department of Chemistry, Iowa State University of Science and Technology, 35A Carver Co-Lab, 1111 Woi Rd., Ames, IA, 50011, USA.
² Ames Laboratory, U.S. Department of Energy, Ames, IA, 50011, USA.
³ Department of Chemistry, Iowa State University of Science and Technology, 35A Carver Co-Lab, 1111 Woi Rd., Ames, IA, 50011, USA. yjlee@iastate.edu.
⁴ Ames Laboratory, U.S. Department of Energy, Ames, IA, 50011, USA. yjlee@iastate.edu.

PMID: 28986913
DOI: 10.1007/978-1-4939-7315-6_13

Abstract

Recent technological advances have pushed the achievable spatial resolution for mass spectrometry imaging (MSI) to cellular and subcellular levels. Direct visualization of maize tissues by this tool has provided key insights into the localization of metabolites and lipids. This chapter outlines methodology for sample preparation, data acquisition, and data analysis of maize tissue sections using high-spatial resolution matrix-assisted laser desorption ionization (MALDI)-MSI, as well as the incorporation of a multi-resolution optical system, which allows for simple inter-conversion between different resolution setups (5, 10, and 50 μm imaging).

Keywords: High-spatial resolution; Lipids; MALDI; Mass spectrometry imaging; Metabolites.

Publication types

Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Image Processing, Computer-Assisted / methods*
Lipids / analysis*
Metabolic Networks and Pathways
Metabolomics / methods*
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods*
Subcellular Fractions / metabolism*
Zea mays / growth & development
Zea mays / metabolism*

Substances

Lipids