SnapShot: Tissue Clearing

Douglas S Richardson; Jeff W Lichtman

doi:10.1016/j.cell.2017.09.025

SnapShot: Tissue Clearing

Cell. 2017 Oct 5;171(2):496-496.e1. doi: 10.1016/j.cell.2017.09.025.

Authors

Douglas S Richardson¹, Jeff W Lichtman²

Affiliations

¹ Harvard Center for Biological Imaging, Harvard University, Cambridge, MA 02138, USA; Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138, USA.
² Harvard Center for Biological Imaging, Harvard University, Cambridge, MA 02138, USA; Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138, USA; Center for Brain Science, Harvard University, Cambridge, MA 02138, USA.

PMID: 28985569
DOI: 10.1016/j.cell.2017.09.025

Abstract

Tissue clearing has become an important tool for the investigation of biological systems in three dimensions. However, many pioneering techniques were based on serendipitous discoveries. Next-generation clearing methods have been (re)designed with a better understanding of the chemistry and physics required to equalize the refractive index throughout a sample which prevents the random bending of light that clouds biological tissues.

MeSH terms

Histological Techniques / methods*
Light
Microscopy / methods*