Characterization and application of porous gold nanoparticles as 2-photon luminescence imaging agents: 20-fold brighter than gold nanorods

Joo H Park; Jisoo Park; Suho Kim; Se-Hwa Kim; Tae G Lee; Jae Y Lee; Jung-Sub Wi

doi:10.1002/jbio.201700174

Characterization and application of porous gold nanoparticles as 2-photon luminescence imaging agents: 20-fold brighter than gold nanorods

J Biophotonics. 2018 Feb;11(2). doi: 10.1002/jbio.201700174. Epub 2017 Oct 29.

Authors

Joo H Park¹, Jisoo Park², Suho Kim², Se-Hwa Kim², Tae G Lee², Jae Y Lee¹, Jung-Sub Wi²

Affiliations

¹ Center for Optical Metrology, Korea Research Institute of Standards and Science, Daejeon, Republic of Korea.
² Center for Nano-Bio Measurement, Korea Research Institute of Standards and Science, Daejeon, Republic of Korea.

PMID: 28976643
DOI: 10.1002/jbio.201700174

Abstract

Two-photon nonlinear microscopy with the aid of plasmonic contrast agents is an attractive bioimaging technique capable of generating high-resolution images in 3 dimensions and facilitating targeted imaging with deep tissue penetration. In this work, physically synthesized gold nanoparticles containing multiple nanopores are used as 2-photon contrast agents and are reported to emit a 20-fold brighter 2-photon luminescence as compared to typical contrast agents, that is, gold nanorods. A successful application of our porous gold nanoparticles is experimentally demonstrated by in vitro nonlinear optical imaging of adipocytes at subcellular level.

Keywords: 2-photon; gold nanorods; luminescence; plasmonic; porous nanoparticles.

Publication types

Comparative Study
Letter
Research Support, Non-U.S. Gov't

MeSH terms

3T3 Cells
Adipocytes / cytology
Contrast Media / chemistry
Gold / chemistry*
Intracellular Space / metabolism
Luminescence*
Metal Nanoparticles*
Mice
Nanotubes*
Optical Imaging / instrumentation
Optical Imaging / methods*
Photons*
Porosity

Substances

Contrast Media
Gold