Abstract
The T4 replisome has provided a unique opportunity to investigate the intricacies of DNA replication. We present a comprehensive review of this system focusing on the following: its 8-protein composition, their individual and synergistic activities, and assembly in vitro and in vivo into a replisome capable of coordinated leading/lagging strand DNA synthesis. We conclude with a brief comparison with other replisomes with emphasis on how coordinated DNA replication is achieved.
Keywords:
DNA helicase; DNA polymerase; DNA primase; DNA replication; DNA-binding protein; bacteriophage.
© 2017 by The American Society for Biochemistry and Molecular Biology, Inc.
MeSH terms
-
Bacteriophage T4 / enzymology
-
Bacteriophage T4 / metabolism*
-
Bacteriophage T7 / enzymology
-
Bacteriophage T7 / metabolism
-
Carbon-Nitrogen Ligases / chemistry
-
Carbon-Nitrogen Ligases / metabolism
-
DNA Replication*
-
DNA-Directed DNA Polymerase / chemistry
-
DNA-Directed DNA Polymerase / metabolism*
-
Escherichia coli / enzymology
-
Escherichia coli / metabolism
-
Escherichia coli Proteins / chemistry
-
Escherichia coli Proteins / metabolism
-
Models, Molecular*
-
Multienzyme Complexes / chemistry
-
Multienzyme Complexes / metabolism*
-
Protein Multimerization
-
Species Specificity
-
Viral Proteins / chemistry
-
Viral Proteins / metabolism*
Substances
-
Escherichia coli Proteins
-
Multienzyme Complexes
-
Viral Proteins
-
DNA synthesome
-
DNA-Directed DNA Polymerase
-
Carbon-Nitrogen Ligases
-
holocarboxylase synthetases