1,2-Dimyristoyl-sn-glycero-3-phosphocholine (DMPC) increases Carmofur stability and in vitro antiproliferative effect

Ilona Domracheva; Ruslan Muhamadejev; Marina Petrova; Edvards Liepinsh; Anita Gulbe; Irina Shestakova; Gunars Duburs; Pavel Arsenyan

doi:10.1016/j.toxrep.2015.01.009

1,2-Dimyristoyl-sn-glycero-3-phosphocholine (DMPC) increases Carmofur stability and in vitro antiproliferative effect

Toxicol Rep. 2015 Jan 27:2:377-383. doi: 10.1016/j.toxrep.2015.01.009. eCollection 2015.

Authors

Ilona Domracheva¹, Ruslan Muhamadejev¹, Marina Petrova¹, Edvards Liepinsh¹, Anita Gulbe¹, Irina Shestakova¹, Gunars Duburs¹, Pavel Arsenyan¹

Affiliation

¹ Latvian Institute of Organic Synthesis, Aizkraukles 21, LV-1006, Riga, Latvia.

Abstract

Addition of DMPC considerably inhibits the degradation of Carmofur in neutral phosphate buffer solutions and this drug becomes less influenced by pH. Carmofur stabilization at neutral pH caused by DMPC addition for in vitro studies was characterized and monitored by ¹H NMR. Antiproliferative activity studies on various tumor cell lines showed considerable increase of Carmofur ability to prevent tumor cell growth, when it is added as a mixture with DMPC. This technique opens a way for Carmofur drug delivery in neutral and basic media.

Keywords: 1,2-Dimyristoyl-sn-glycero-3-phosphocholine (PubChem CID: 5459377); 1-Hexylcarbamoyl-5-fluorouracil (PubChem CID: 2577); 1H NMR; 5-Fluorouracil; 5-Fluorouracil (PubChem CID: 3385); 5-Fu, 5-fluorouracil; Carmofur; Carmofur, 1-hexylcarbamoyl-5-fluorouracil; Cytotoxicity; DMPC; DMPC, 1,2-dimyristoyl-sn-glycero-3-phosphocholine; Drug delivery; NMR, nuclear magnetic resonance.