Development and Observation of Mature Megagametophyte Cell-Specific Fluorescent Markers

Methods Mol Biol. 2017:1669:55-65. doi: 10.1007/978-1-4939-7286-9_5.

Abstract

Visualization of the intact embryo sac within the ovular/gynoecial tissues and clear identification of cell types can be logistically difficult and subject to interpretation. Cellular marker technologies have been available for the embryo sac, but have typically labeled only one cell type in a particular line. Here, we describe techniques for simultaneous labeling each cell type in the embryo sac and visualization methods for such in Arabidopsis, soybean, maize, and sorghum.

Keywords: Cell labeling; Cell visualization; Embryo sac; Fluorescent reporter; Megagametophyte.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Arabidopsis / metabolism*
  • Arabidopsis Proteins / metabolism
  • Gene Expression Regulation, Plant / physiology
  • Ovule / metabolism

Substances

  • Arabidopsis Proteins