Over-expression of PPARα in obese mice adipose tissue improves insulin sensitivity

Haruya Takahashi; Kohei Sanada; Hiroyuki Nagai; Yongjia Li; Yumeko Aoki; Takeshi Ara; Shigeto Seno; Hideo Matsuda; Rina Yu; Teruo Kawada; Tsuyoshi Goto

doi:10.1016/j.bbrc.2017.09.067

Over-expression of PPARα in obese mice adipose tissue improves insulin sensitivity

Biochem Biophys Res Commun. 2017 Nov 4;493(1):108-114. doi: 10.1016/j.bbrc.2017.09.067. Epub 2017 Sep 15.

Authors

Affiliations

¹ Laboratory of Molecular Function of Food, Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University, Uji, Kyoto, Japan.
² Gifu Prefectural Research Institute for Health and Environmental Science, Gifu, Japan.
³ Graduate School of Information Science and Technology, Osaka University, Osaka, Japan.
⁴ Department of Food Science and Nutrition, University of Ulsan, Ulsan, South Korea.
⁵ Laboratory of Molecular Function of Food, Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University, Uji, Kyoto, Japan; Research Unit for Physiological Chemistry, Kyoto University, Kyoto, Japan.
⁶ Laboratory of Molecular Function of Food, Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University, Uji, Kyoto, Japan; Research Unit for Physiological Chemistry, Kyoto University, Kyoto, Japan. Electronic address: tgoto@kais.kyoto-u.ac.jp.

PMID: 28919422
DOI: 10.1016/j.bbrc.2017.09.067

Abstract

Peroxisome proliferator-activated receptor α (PPARα) is important in the regulation of lipid metabolism and expressed at high levels in the liver. Although PPARα is also expressed in adipose tissue, little is known about the relationship between its activation and the regulation of glucose metabolism. In this study, we developed adipose tissue specific PPARα over-expression (OE) mice. Metabolomics and insulin tolerance tests showed that OE induces branched-chain amino acid (BCAA) profile and improvement of insulin sensitivity. Furthermore, LC-MS and PCR analyses revealed that OE changes free fatty acid (FFA) profile and reduces obesity-induced inflammation. These findings suggested that PPARα activation in adipose tissue contributes to the improvement of glucose metabolism disorders via the enhancement of BCAA and FFA metabolism.

Keywords: Adipose tissue; Insulin sensitivity; PPARα.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adipose Tissue / metabolism*
Animals
Blood Glucose / metabolism*
Fatty Acids, Nonesterified / metabolism*
Insulin / metabolism*
Insulin Resistance*
Male
Mice
Mice, Inbred C57BL
Obesity / metabolism*
PPAR alpha / metabolism*
Up-Regulation

Substances

Blood Glucose
Fatty Acids, Nonesterified
Insulin
PPAR alpha