Skin regeneration effect of the Glycosaminoglycans from Liparis tessellatus eggs

Jae-Joon Lee; Bernadeth F Ticar; Zuliyati Rohmah; Si-Hyang Park; Dawon Kang; Seok-Joong Kang; Byeong-Dae Choi

doi:10.1016/j.ijbiomac.2017.08.182

Skin regeneration effect of the Glycosaminoglycans from Liparis tessellatus eggs

Int J Biol Macromol. 2017 Dec;105(Pt 2):1369-1374. doi: 10.1016/j.ijbiomac.2017.08.182. Epub 2017 Sep 14.

Authors

Jae-Joon Lee¹, Bernadeth F Ticar², Zuliyati Rohmah³, Si-Hyang Park⁴, Dawon Kang⁵, Seok-Joong Kang⁶, Byeong-Dae Choi⁷

Affiliations

¹ Department of Food and Nutrition, Chosun University, Gwangju 61452, Republic of Korea. Electronic address: leejj80@chosun.ac.kr.
² Department of Seafood and Aquatic Science, The Institute of Marine Industry, Gyeongsang National University, Tongyeong 53210, Republic of Korea; College of Arts and Sciences, Iloilo Science and Technology University, Burgos St., La Paz, Iloilo City 5000, Iloilo, Philippines. Electronic address: berna2423@gmail.com.
³ Department of Seafood and Aquatic Science, The Institute of Marine Industry, Gyeongsang National University, Tongyeong 53210, Republic of Korea; Faculty of Biology, Universitas Gadjah Mada, Yogyakarta 55281, Indonesia. Electronic address: rohmah.z@gmail.com.
⁴ Sunmarine Biotech Co., Tongyeong 53210, Republic of Korea.
⁵ Department of Physiology, College of Medicine and Institute of Health Sciences, Gyeongsang National University, Jinju 52727, Republic of Korea.
⁶ Department of Seafood and Aquatic Science, The Institute of Marine Industry, Gyeongsang National University, Tongyeong 53210, Republic of Korea.
⁷ Department of Seafood and Aquatic Science, The Institute of Marine Industry, Gyeongsang National University, Tongyeong 53210, Republic of Korea. Electronic address: bdchoi@gnu.ac.kr.

PMID: 28917941
DOI: 10.1016/j.ijbiomac.2017.08.182

Abstract

A purified Glycosaminoglycans (GAGs) extract from L. tessellatus eggs was enzyme-hydrolyzed and then fractionated with DEAE-Sepharose column chromatography. The fractions were subsequently subjected to skin regeneration effects analysis against skin fibroblast (CCD-986sk) cell lines. Fraction 3 is evidently to have tyrosinase inhibition activity by 20.1% at concentration of 50mg/mL. DOPA oxidation assay, collagenase inhibition activity assay, fibroblast proliferation assay, and production of type I C-peptide assays were done to further proof the skin regeneration effect of GAGs fractions. Results revealed that fraction 3 has effective skin regeneration activities at a concentration of 200mg/mL.

Keywords: Fibroblast proliferation; Glycosaminoglycans; Liparis tessellatus eggs; Tyrosinase inhibition.

MeSH terms

Animals
Cell Line
Cell Proliferation / drug effects
Collagen Type I / biosynthesis
Collagenases / metabolism
Fibroblasts / cytology
Fibroblasts / drug effects
Fishes*
Glycosaminoglycans / pharmacology*
Monophenol Monooxygenase / antagonists & inhibitors
Ovum / chemistry*
Regeneration / drug effects*
Skin / cytology
Skin / drug effects*

Substances

Collagen Type I
Glycosaminoglycans
Monophenol Monooxygenase
Collagenases