Isolation of a 2-picolinic acid-assimilating bacterium and its proposed degradation pathway

Chunli Zheng; Qiaorui Wang; Yanli Ning; Yurui Fan; Shanshan Feng; Chi He; Tian C Zhang; Zhenxing Shen

doi:10.1016/j.biortech.2017.09.031

Isolation of a 2-picolinic acid-assimilating bacterium and its proposed degradation pathway

Bioresour Technol. 2017 Dec;245(Pt A):681-688. doi: 10.1016/j.biortech.2017.09.031. Epub 2017 Sep 6.

Authors

Chunli Zheng¹, Qiaorui Wang¹, Yanli Ning², Yurui Fan¹, Shanshan Feng¹, Chi He³, Tian C Zhang⁴, Zhenxing Shen¹

Affiliations

¹ Department of Environmental Science and Engineering, State Key Laboratory of Multiphase Flow in Power Engineering, School of Energy and Power Engineering, Xi'an Jiaotong University, Xi'an 710049, Shaanxi, PR China.
² Xi'an Modern Chemistry Research Institute, Xi'an 710065, PR China.
³ Department of Environmental Science and Engineering, State Key Laboratory of Multiphase Flow in Power Engineering, School of Energy and Power Engineering, Xi'an Jiaotong University, Xi'an 710049, Shaanxi, PR China; School of Chemistry, Cardiff University, Main Building, Park Place, Cardiff CF10 3AT, UK. Electronic address: chi_he@xjtu.edu.cn.
⁴ 205D, PKI, Civil Engineering Department, University of Nebraska-Lincoln at Omaha campus, Omaha, NE 68182-0178, USA.

PMID: 28917103
DOI: 10.1016/j.biortech.2017.09.031

Abstract

Burkholderia sp. ZD1, aerobically utilizes 2-picolinic acid as a source of carbon, nitrogen and energy, was isolated. ZD1 completely degraded 2-picolinic acid when the initial concentrations ranged from 25 to 300mg/L. Specific growth rate (μ) and specific consumption rate (q) increased continually in the concentration range of 25-100mg/L, and then declined. Based on the Haldane model and Andrew's model, μ_max and q_max were calculated as 3.9 and 16.5h^-1, respectively. Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR-MS) was used to determine the main intermediates in the degradation pathway. Moreover, attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR) was innovatively used to deduce the ring cleavage mechanism of N-heterocycle of 2-picolinic acid. To our knowledge, this is the first report on not only the utilization of 2-picolinic acid by a Burkholderia sp., but also applying FT-ICR-MS and ATR-FTIR for exploring the biodegradation pathway of organic compounds.

Keywords: 2-Picolinic acid; ATR-FTIR; Biodegradation pathway; Burkholderia sp.; FT-ICR-MS.

MeSH terms

Biodegradation, Environmental*
Fourier Analysis
Mass Spectrometry
Metabolic Networks and Pathways
Picolinic Acids*

Substances

Picolinic Acids