Biolistic Transformation of Wheat

Caroline Tassy; Pierre Barret

doi:10.1007/978-1-4939-7337-8_9

Biolistic Transformation of Wheat

Methods Mol Biol. 2017:1679:141-152. doi: 10.1007/978-1-4939-7337-8_9.

Authors

Caroline Tassy¹, Pierre Barret²

Affiliations

¹ INRA, UMR GDEC, Genetic, Diversity and Ecophysiology of Cereals, 5, Chemin de Beaulieu, 63000, Clermont Ferrand, France. caroline.tassy@inra.fr.
² INRA, UMR GDEC, Genetic, Diversity and Ecophysiology of Cereals, 5, Chemin de Beaulieu, 63000, Clermont Ferrand, France.

PMID: 28913799
DOI: 10.1007/978-1-4939-7337-8_9

Abstract

The wheat genome encodes some 100,000 genes. To understand how the expression of these genes is regulated it will be necessary to carry out many genetic transformation experiments. Robust protocols that allow scientists to transform a wide range of wheat genotypes are therefore required. In this chapter, we describe a protocol for biolistic transformation of wheat that uses immature embryos and small quantities of DNA cassettes. An original method for DNA cassette purification is also described. This protocol can be used to transform a wide range of wheat genotypes and other related species.

Keywords: Biolistic transformation; Gene cassette; Wheat.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Biolistics / methods*
In Vitro Techniques
Plants, Genetically Modified
Seeds / genetics
Transformation, Genetic*
Triticum / genetics*