Ultra-High Efficient Colony PCR for High Throughput Screening of Bacterial Genes

Mohammad Abu Hena Mostofa Jamal; Satya Priya Sharma; Hea-Jong Chung; Hyeon-Jin Kim; Seong-Tshool Hong; Seungkoo Lee

doi:10.1007/s12088-017-0665-1

Ultra-High Efficient Colony PCR for High Throughput Screening of Bacterial Genes

Indian J Microbiol. 2017 Sep;57(3):365-369. doi: 10.1007/s12088-017-0665-1. Epub 2017 Aug 10.

Authors

Mohammad Abu Hena Mostofa Jamal^#^{1

2}, Satya Priya Sharma^#¹, Hea-Jong Chung^{1

3}, Hyeon-Jin Kim⁴, Seong-Tshool Hong¹, Seungkoo Lee⁵

Affiliations

¹ Department of Biomedical Sciences and Institute for Medical Science, Chonbuk National University Medical School, Chonbuk National University, Jeonju, South Korea.
² Department of Biotechnology and Genetic Engineering, Islamic University, Kushtia, Bangladesh.
³ Department of Microbiology, Seonam University Medical School, Namwon, Chonbuk 55321 South Korea.
⁴ JINIS BDRD Institute, JINIS Biopharmaceuticals Co., Wanju, Chonbuk Korea.
⁵ Department of Anatomic Pathology, School of Medicine, Kangwon National University Hospital, Kanwong National University, Chuncheon, Gangwon 24341 South Korea.

^# Contributed equally.

Abstract

Current colony PCR methods are not suitable for screening genes encoded in genomic DNA and are limited to E. coli host strains. Here, we describe an ultra-high efficient colony PCR method for high throughput screening of bacterial genes embedded in the genomic DNA of any bacterial species. This new technique expands colony PCR method to several hosts as well as offers a rapid, less expensive and reliable bacterial genomic DNA extraction.

Keywords: Colony PCR; DNA extraction; High throughput screening; Sequencing; Sonication.