Production of highly immunogenic virus-like particles of bovine papillomavirus type 6 in silkworm pupae

Vaccine. 2017 Oct 13;35(43):5878-5882. doi: 10.1016/j.vaccine.2017.08.079. Epub 2017 Sep 8.

Abstract

Bovine papillomaviruses (BPVs) are the causative agent of bovine teat papillomatosis, which can lead to severe economic losses in dairy cattle. Among the 14 identified BPV genotypes, BPV type 6 (BPV6) is the most frequently detected in teat papilloma lesions, and is therefore thought to play a major role in teat papillomatosis. To develop an effective vaccine against BPV6 infection, we produced virus-like particles of BPV6 (BPV6-VLP) in silkworm (Bombyx mori) pupae and purified these by heparin affinity chromatography using a single column. About 0.7mg purified BPV6-VLP was obtained from one pupa. BPV6-VLP-immunized mice produced a specific IgG to BPV6 that recognized BPV6 antigen with high sensitivity in an immunohistochemical analysis. Thus, silkworm pupae are a useful bioreactor for the production of BPV6-VLP, which can potentially be used as a vaccine for bovine teat papillomatosis.

Keywords: Bovine papilloma virus type 6; Silkworm; Teat papillomatosis; Vaccine; Virus-like particle.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antigens, Viral
  • Bombyx / immunology*
  • Cattle
  • Cattle Diseases / immunology*
  • Cattle Diseases / prevention & control
  • Cattle Diseases / virology
  • Female
  • Genotype
  • Mice
  • Mice, Inbred BALB C
  • Papilloma / immunology*
  • Papilloma / prevention & control
  • Papillomaviridae / immunology*
  • Papillomavirus Infections / immunology*
  • Papillomavirus Infections / prevention & control
  • Pupa / immunology*
  • Vaccination / methods
  • Vaccines, Virus-Like Particle / immunology*

Substances

  • Antigens, Viral
  • Vaccines, Virus-Like Particle