Genome shuffling improved the nucleosides production in Cordyceps kyushuensis

Yanming Wang; Guoying Zhang; Xuan Zhao; Jianya Ling

doi:10.1016/j.jbiotec.2017.08.021

Genome shuffling improved the nucleosides production in Cordyceps kyushuensis

J Biotechnol. 2017 Oct 20:260:42-47. doi: 10.1016/j.jbiotec.2017.08.021. Epub 2017 Sep 4.

Authors

Yanming Wang¹, Guoying Zhang², Xuan Zhao¹, Jianya Ling³

Affiliations

¹ State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, China.
² Shandong University of Traditional Chinese Medicine, Jinan 250014, China.
³ State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, China. Electronic address: lingjianya2014@126.com.

PMID: 28882571
DOI: 10.1016/j.jbiotec.2017.08.021

Abstract

Genome shuffling was first applied to improve the production of nucleosides in Cordyceps kyushuensis. Six improved strains were selected for genome shuffling by UV and HNO₂ mutagenesis. Ten improved genome shuffling strains with good genetic stability were obtained, among which, the production of cordycepin in R6 was 9.624 times higher than that of the ancestor. While in R18 and R19, the yield of cordycepin, adenosine, guanosine and uridine were all increased greatly compared with the ancestor. Based on the four phenotypes of the content of cordycepin, adenosine, guanosine and uridine, hierarchical clustering analysis (HCA) and principal component analysis (PCA) were applied to infer the relationships between genome shuffling strains and mutants.

Keywords: Cordyceps kyushuensis; Genome shuffling; Nucleosides; Production.

MeSH terms

Cordyceps / genetics*
Cordyceps / metabolism*
DNA Shuffling / methods*
Metabolic Engineering / methods*
Nucleosides / analysis
Nucleosides / metabolism*
Principal Component Analysis

Substances

Nucleosides