Objective: To study the potential of differentiation of menstrual blood-derived mesenchymal stem cells (MenSC) into endometrial epithelial cells (EEC) in vitro. Methods: Endometrial stromal cells (ESC) and MenSC were cultured and identified by certain methods.MenSC were co-cultured with ESC, added 10 ng/ml TGF-β1, 10 ng/ml EGF, 10 ng/ml PDGF-BB and 1×10(-7) mol/L 17-β-estradiol.The expression of cytokeratin, which was marker of EEC, was tested by immunofluorescence in differentiated MenSC. Results: MenSC proliferated quickly in vitro, and showed fusiform shape and clear structure within 10 generations.The expression rate of superficial markers of MenSC, CD44, CD90, CD73, and CD29, were 98.4%, 90.77%, 94.75% and 97.01% respectively.The expression of vimentin, which was stromal marker, was positive in cultured ESC (P3). After co-cultured with ESC for 3 weeks, MenSC morphology changed from fibroblast-like cells into epithelial-like cells.Immunofluorescence assay showed that the differentiated MenSC expressed cytokeratin. Conclusion: Under the condition of co-culture with ESC, MenSC has the ability to differentiate into endometrial epithelial cells. Cell growth factor and estrogen play the important roles in this process.
目的： 体外诱导月经血子宫内膜间充质干细胞(MenSC)向子宫内膜上皮细胞分化。 方法： 培养MenSC和子宫内膜间质细胞(ESC)并鉴定，将MenSC与ESC共培养，同时补充生长因子(TGF－β1 10 ng/ml，EGF 10 ng/ml，PDGF－BB 10 ng/ml)和雌激素(17－β－雌二醇1×10(－7) mol/L)，之后免疫荧光染色检测分化的MenSC是否表达上皮细胞特异标记细胞角蛋白(CK)。 结果： MenSC生长良好，10代以内均表现出明显的梭状形态，结构紧密，其表面标记CD44、CD90、CD73和CD29表达率分别为98.4%、90.77%、94.75%和97.01%。ESC特异标记波形蛋白表达阳性。MenSC与ESC共培养3周后细胞形态基本由成纤维样向上皮样细胞转变，免疫荧光鉴定诱导分化后的MenSC表达细胞角蛋白。 结论： 在ESC共培养环境的诱导下，MenSC可向子宫内膜上皮细胞分化。细胞生长因子和雌激素在这一进程中发挥重要作用。.
Keywords: Differentiation; Endometrial injury; Endometrial stromal cells; Mesenchymal stem cells.