The lipopolysaccharide transport (Lpt) machinery: A nonconventional transporter for lipopolysaccharide assembly at the outer membrane of Gram-negative bacteria

J Biol Chem. 2017 Nov 3;292(44):17981-17990. doi: 10.1074/jbc.R117.802512. Epub 2017 Sep 6.

Abstract

The outer membrane (OM) of Gram-negative is a unique lipid bilayer containing LPS in its outer leaflet. Because of the presence of amphipathic LPS molecules, the OM behaves as an effective permeability barrier that makes Gram-negative bacteria inherently resistant to many antibiotics. This review focuses on LPS biogenesis and discusses recent advances that have contributed to our understanding of how this complex molecule is transported across the cellular envelope and is assembled at the OM outer leaflet. Clearly, this knowledge represents an important platform for the development of novel therapeutic options to manage Gram-negative infections.

Keywords: ABC transporter; LPS transport; gram-negative bacteria; lipopolysaccharide (LPS); outer membrane; protein-protein interaction.

Publication types

  • Review
  • Research Support, Non-U.S. Gov't

MeSH terms

  • ATP-Binding Cassette Transporters / chemistry
  • ATP-Binding Cassette Transporters / metabolism
  • Bacterial Outer Membrane Proteins / chemistry
  • Bacterial Outer Membrane Proteins / metabolism*
  • Bacterial Proteins / chemistry
  • Bacterial Proteins / metabolism
  • Biological Transport
  • Carbohydrate Conformation
  • Carrier Proteins / chemistry
  • Carrier Proteins / metabolism
  • Gram-Negative Bacteria / metabolism*
  • Lipopolysaccharides / biosynthesis
  • Lipopolysaccharides / chemistry
  • Lipopolysaccharides / metabolism*
  • Models, Biological*
  • Protein Conformation

Substances

  • ATP-Binding Cassette Transporters
  • Bacterial Outer Membrane Proteins
  • Bacterial Proteins
  • Carrier Proteins
  • Lipopolysaccharides
  • MsbA protein, Bacteria

Associated data

  • PDB/5X5Y
  • PDB/3M2Y
  • PDB/2R19
  • PDB/5IV9