Large-scale immuno-magnetic cell sorting of T cells based on a self-designed high-throughput system for potential clinical application

Qian Zhang; Ting Yin; Rongrong Xu; Wenjun Gao; Hui Zhao; Joseph G Shapter; Kan Wang; Yulan Shen; Peng Huang; Guo Gao; Yanfeng Wu; Daxiang Cui

doi:10.1039/c7nr04914e

Large-scale immuno-magnetic cell sorting of T cells based on a self-designed high-throughput system for potential clinical application

Nanoscale. 2017 Sep 21;9(36):13592-13599. doi: 10.1039/c7nr04914e.

Authors

Qian Zhang¹, Ting Yin¹, Rongrong Xu², Wenjun Gao³, Hui Zhao³, Joseph G Shapter⁴, Kan Wang¹, Yulan Shen⁵, Peng Huang¹, Guo Gao¹, Yanfeng Wu², Daxiang Cui¹

Affiliations

¹ Institute of Nano Biomedicine and Engineering, Shanghai Engineering Research Center for Intelligent diagnosis and treatment instrument, Department of Instrument Science and Engineering, School of Electronic Information and Electrical Engineering, Shanghai Jiao Tong University, 800 Dongchuan Rd, Shanghai 200240, China. guogao@sjtu.edu.cn dxcui@sjtu.edu.cn.
² National Key Laboratory of Medical Immunology, Institute of Immunology, Second Military Medical University, Shanghai 200433, China. wuyf@immunol.org.
³ Department of Instrument Science and Engineering, School of Electronic Information and Electrical Engineering, Shanghai Jiao Tong University, 800 Dongchuan Rd, Shanghai 200240, China.
⁴ School of Chemical and Physical Sciences, Flinders University, Bedford Park, Adelaide 5042, Australia.
⁵ Department of Radiology, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai JiaoTong University, Shanghai, 200240, China.

PMID: 28875998
DOI: 10.1039/c7nr04914e

Abstract

In this contribution, we designed four types of immuno-magnetic nanoparticles for separation of different T cells (CD3⁺, CD4⁺, CD8⁺ and CD14⁺ T cells), and we established a new large-scale immuno-magnetic cell sorting procedure to achieve an enrichment of particular T cells using our designed auto-IMACS device. This device could achieve recyclable large-scale cell sorting, for which the throughput of the system reached ∼4000 mL and the maximum cell capacity was 4 × 10¹⁰. The collected cells were analyzed by flow cytometry and visual cytology data, and the effective selection rates of CD3⁺, CD4⁺, CD8⁺ and CD14⁺ T cells were 79.3%, 74.1%, 57.1% and 67.9%, respectively. The sorted CD8⁺ T cells still retained good cytotoxic activity against specific cells. In addition, the sorted T cells can also be further incubated in vitro and proliferated, and even could be infused back into patients for immunotherapy in the near future.

MeSH terms

Cell Separation*
Flow Cytometry*
Humans
Magnetics
T-Lymphocytes / cytology*