New furostanol saponins with anti-inflammatory and cytotoxic activities from the rhizomes of Smilax davidiana

Steroids. 2017 Nov:127:62-68. doi: 10.1016/j.steroids.2017.08.013. Epub 2017 Sep 1.

Abstract

Seven new furostanol saponins have been isolated from the rhizomes of Smilax davidiana. Their structures were established by 2D NMR spectroscopic techniques (1H,1H-COSY, NOESY, HSQC and HMBC), mass spectrometry and comparison with the literature. The isolated compounds were subjected to evaluate anti-inflammatory and cytotoxic activities in vitro. Compounds 3, 5 and 7 were found to have modest anti-inflammatory effects through suppression of IL-1β production and promote the expression of IL-10 in LPS-stimulated RAW 264.7 cells. Davidianoside F (6) showed activity against MCF-7 and HELA cell lines at the concentration of 10.2µM and 4.3µM, respectively.

Keywords: Anti-inflammatory; Cytotoxic activities; Furostanol saponins; Smilax davidiana.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Anti-Inflammatory Agents / chemistry
  • Anti-Inflammatory Agents / pharmacology
  • Antineoplastic Agents / chemistry
  • Antineoplastic Agents / pharmacology
  • Gene Expression Regulation / drug effects
  • HeLa Cells
  • Humans
  • Interleukin-10 / metabolism
  • Interleukin-1beta / biosynthesis
  • MCF-7 Cells
  • Mice
  • RAW 264.7 Cells
  • Rhizome / chemistry*
  • Saponins / chemistry*
  • Saponins / pharmacology*
  • Smilax / chemistry*

Substances

  • Anti-Inflammatory Agents
  • Antineoplastic Agents
  • Interleukin-1beta
  • Saponins
  • Interleukin-10