Determination of the 99th percentile upper reference limit for high-sensitivity cardiac troponin I in Malaysian population

Abstract

Introduction of high-sensitivity cardiac troponin I (hscTn I) assays for routine clinical use in Malaysia requires determination of the 99^th percentile upper reference limit (URL) for each assay to suit local context. Hence, this study aimed to determine the 99^th percentile URL for hscTn I in the Malaysian population. A total of 250 (120 males and 130 females) healthy Malaysian blood donors aged 18 to 60 years old were recruited. Blood samples for hscTn I were measured using Abbott Diagnostics hscTn I assay on Architect i2000sr analyser. The 99^th percentile was calculated using a non-parametric method and gender specific results were compared. The 99^th percentile URL for hscTn I for the overall population was 23.7 ng/L, with gender specific values being 29.9 ng/L and 18.6 ng/L for male and female, respectively. Females had significantly lower hscTn I compared to males. This study confirms the use of gender specific 99^th percentile URL for hscTn I for clinical use in a multi-ethnic Malaysian population.