Comparative analysis of expression of genes encoding enzymes of catecholamine catabolism (monoaminbe oxidases A and B (MAO A and MAO B) and catechol-O-methyl transferase (COMT)) and renalase has been carried out in tissues of normotensive Wistar-Kyoto (WKY) rats and spontaneously hypertensive rats (SHR). Among investigated tissues the highest level of mRNA of genes encoding key enzymes of catecholamine catabolism (MAO A, MAO B, COMT) was found in the heart of WKY rats. In SHR the mRNA levels of these genes were lower (p<0.05-0.01), however, no similar changes were observed in the tissues studied in dependence of hypertension. The relative mRNA levels of the studied genes normalized versus actin mRNA significantly varied. In heart and kidney the relative level of COMT mRNA significantly exceeded the relative levels of both MAO A mRNA and MAO B mRNA. In the brain differences in mRNAs of MAOA, MAOB, and COMT were less pronounced. However, in all examined tissue the renalase mRNA level was much (at least 10-20-fold) lower than any other mRNA studied. Taking into consideration known correlations between mRNAs and corresponding protein products reported in the literature for many genes these results suggest that in the case of any catalytic scenarios proposed or even proved for renalase this protein cannot contribute to catecholamine degradation. It is also unlikely that the products of renalase reaction, b-NAD(P)+ and hydrogen peroxide, can exhibit a hypotensive effect due to low expression of the renalase encoding gene.
Proveden sravnitel'nyĭ analiz ékspressii genov, kodiruiushchikh fermenty katabolizma katekholaminov (monoaminoksidazy A i B (MAO A i MAO B), katekhol-O-metiltransferazu (KOMT)) i renalazu v tkaniakh mozga, serdtsa i pochek krys so spontannoĭ gipertenzieĭ (SHR) i kontrol'nykh normotenzivnykh krys Wistar-Kyoto (WKY). Sredi issledovannykh tkaneĭ uroven' mRNK genov, kodiruiushchikh kliuchevye fermenty degradatsii katekholaminov (MAO A, MAO B, KOMT), byl naivysshim v serdtse normotenzivnykh krys WKY. U krys SHR uroven' mRNK étikh genov byl nizhe (r<0,05-0,01), odnako skhodnykh izmeneniĭ v issledovannykh tkaniakh v zavisimosti ot urovnia gipertenzii otmecheno ne bylo. Otnositel'nyĭ uroven' ékspressii mRNK issledovannykh genov, normirovannyĭ po urovniu mRNK aktina, var'iroval v dovol'no shirokikh predelakh. V serdtse i pochkakh otnositel'nyĭ uroven' mRNK KOMT znachitel'no prevyshal otnositel'nyĭ uroven' mRNK i MAO A, i MAO B. V mozge razlichiia v urovniakh mRNK MAO A, mRNK MAO B i mRNK KOMT byli ne stol' vyrazheny. Odnako vo vsekh izuchennykh tkaniakh uroven' mRNK renalazy byl namnogo (v 10-20 i bolee raz) nizhe liubogo iz mRNK issledovannykh genov. S uchetom izvestnykh korreliatsiĭ mezhdu urovnem mRNK i sootvetstvuiushchego belka-produkta, vyiavlennykh dlia mnogikh genov, éto, ochevidno, svidetel'stvuet o tom, chto pri realizatsii liubogo iz kataliticheskikh stsenariev, “napisannykh” v raznoe vremia dlia renalazy, dannyĭ belok ne mozhet vnosit' skol'ko-nibud' sushchestvennogo vklada v degradatsiiu katekholaminov. Predstavliaetsia takzhe maloveroiatnym, chto i produkty renalaznoĭ reaktsii – b-NAD(P)+ i N2O2 – mogut okazyvat' gipotenzivnoe deĭstvie iz-za nizkogo urovnia ékspressii kodiruiushchego renalazu gena.
Keywords: SHR and WKY rats; gene expression; genes encoding monoamine oxidases and catechol-O-methyl transferase; renalase.