1H, 13C and 15N resonance assignment of human guanylate kinase

Nazimuddin Khan; David Ban; Pablo Trigo-Mourino; Marta G Carneiro; Manfred Konrad; Donghan Lee; T Michael Sabo

doi:10.1007/s12104-017-9771-6

¹H, ¹³C and ¹⁵N resonance assignment of human guanylate kinase

Biomol NMR Assign. 2018 Apr;12(1):11-14. doi: 10.1007/s12104-017-9771-6. Epub 2017 Aug 31.

Authors

Nazimuddin Khan^{1

2}, David Ban¹, Pablo Trigo-Mourino³, Marta G Carneiro^{3

4}, Manfred Konrad², Donghan Lee^{1

3}, T Michael Sabo^{5

6}

Affiliations

¹ James Graham Brown Cancer Center, Department of Medicine, University of Louisville, 505 S. Hancock St., Louisville, KY, 40202, USA.
² Enzyme Biochemistry Group, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077, Göttingen, Germany.
³ Department for NMR-Based Structural Biology, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077, Göttingen, Germany.
⁴ ZoBio B.V., Biopartner building 2, J.H. Oortweg 19, 2333 CH, Leiden, The Netherlands.
⁵ James Graham Brown Cancer Center, Department of Medicine, University of Louisville, 505 S. Hancock St., Louisville, KY, 40202, USA. mike.sabo@louisville.edu.
⁶ Department for NMR-Based Structural Biology, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077, Göttingen, Germany. mike.sabo@louisville.edu.

PMID: 28861857
DOI: 10.1007/s12104-017-9771-6

Abstract

Human guanylate kinase (hGMPK) is a critical enzyme that, in addition to phosphorylating its physiological substrate (d)GMP, catalyzes the second phosphorylation step in the conversion of anti-viral and anti-cancer nucleoside analogs to their corresponding active nucleoside analog triphosphates. Until now, a high-resolution structure of hGMPK is unavailable and thus, we studied free hGMPK by NMR and assigned the chemical shift resonances of backbone and side chain ¹H, ¹³C, and ¹⁵N nuclei as a first step towards the enzyme's structural and mechanistic analysis with atomic resolution.

Keywords: Enzyme; Guanylate kinase (GMPK); NMR assignment; Nucleotide kinase.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Guanylate Kinases / chemistry*
Humans
Nuclear Magnetic Resonance, Biomolecular*

Substances

Guanylate Kinases