Knockdown of REGγ inhibits proliferation by inducing apoptosis and cell cycle arrest in prostate cancer

Shaojun Chen; Longsheng Wang; Chen Xu; Hui Chen; Bo Peng; Yunfei Xu; Xudong Yao; Lei Li; Junhua Zheng

Knockdown of REGγ inhibits proliferation by inducing apoptosis and cell cycle arrest in prostate cancer

Am J Transl Res. 2017 Aug 15;9(8):3787-3795. eCollection 2017.

Authors

Shaojun Chen¹, Longsheng Wang¹, Chen Xu¹, Hui Chen², Bo Peng¹, Yunfei Xu¹, Xudong Yao¹, Lei Li², Junhua Zheng¹

Affiliations

¹ Department of Urology, Shanghai Tenth People's Hospital, Tongji UniversityShanghai, 200072, China.
² Shanghai Key Laboratory of Regulatory Biology, Institute of Biomedical Sciences, School of Life Sciences, East China Normal UniversityShanghai, 200241, China.

PMID: 28861169
PMCID: PMC5575192

Abstract

Prostate cancer (PCa) is the most common malignant tumor and the second leading cause of cancer related death among men in western countries. REGγ, a proteasome activator, is reported to play important roles in various human cancers. However, the expression patterns and potential roles of REGγ in prostate cancer have never been reported. In this study, we found for the first time that REGγ is overexpressed in prostate cancer tissues and cell lines at both protein and mRNA levels. In addition, we demonstrated that knockdown of REGγ significantly inhibited cell proliferation and induced apoptosis and cell cycle arrest in PCa cell lines PC-3 and DU145. Moreover, we observed that the expressions of P21 were increased while the levels of cycinD1 and bcl-2 were decreased after knockdown of REGγ in PCa cells. And the stabilization of P21 was enhanced after REGγ knockdown in PC-3 cells. In summary, our findings suggest that REGγ may play important roles in prostate cancer and may serve as a novel therapeutic target in the treatment of PCa patients.

Keywords: Prostate cancer; REGγ; apoptosis; cell cycle; proliferation.