Differential gene expression in leaf tissues between mutant and wild-type genotypes response to late leaf spot in peanut (Arachis hypogaea L.)

Suoyi Han; Hua Liu; Mei Yan; Feiyan Qi; Yaqi Wang; Ziqi Sun; Bingyan Huang; Wenzhao Dong; Fengshou Tang; Xinyou Zhang; Guohao He

doi:10.1371/journal.pone.0183428

Differential gene expression in leaf tissues between mutant and wild-type genotypes response to late leaf spot in peanut (Arachis hypogaea L.)

PLoS One. 2017 Aug 25;12(8):e0183428. doi: 10.1371/journal.pone.0183428. eCollection 2017.

Authors

Suoyi Han^{1

2}, Hua Liu¹, Mei Yan¹, Feiyan Qi¹, Yaqi Wang³, Ziqi Sun³, Bingyan Huang³, Wenzhao Dong⁴, Fengshou Tang⁴, Xinyou Zhang⁴, Guohao He²

Affiliations

¹ Industrial Crops Research Institute, Henan Academy of Agricultural Sciences, Zhengzhou, Henan, China.
² Department of Agricultural and Environmental Sciences, College of Agriculture, Environment and Nutrition Sciences, Tuskegee University, Tuskegee, Alabama, United States of America.
³ Henan Provincial Key Laboratory for Oil Crops Improvement, Zhengzhou, Henan, China.
⁴ Key Laboratory of Oil Crops in Huanghuaihai Plains, Ministry of Agriculture, Zhengzhou, Henan, China.

Abstract

Late leaf spot (LLS) is a major foliar disease in peanut (A. hypogaea L.) worldwide, causing significant losses of potential yield in the absence of fungicide applications. Mutants are important materials to study the function of disease-related genes. In this study, the mutant line M14 was derived from cultivar Yuanza 9102 treated with EMS. Yuanza 9102 was selected from an interspecific cross of cultivar Baisha 1016 with A. diogoi, and is resistant to several fungal diseases. By contrast, the M14 was highly susceptible to late leaf spot. RNA-Seq analysis in the leaf tissues of the M14 and its wild type Yuanza 9102 under pathogen challenge showed 2219 differentially expressed genes including1317 up-regulated genes and 902 down-regulated genes. Of these genes, 1541, 1988, 1344, 643 and 533 unigenes were obtained and annotated by public protein databases of SwissPort, TrEMBL, gene ontology (GO), KEGG and clusters of orthologous groups (COG), respectively. Differentially expressed genes (DEGs) showed that expression of inducible pathogenesis-related (PR) proteins was significantly up-regulated; in the meantime DEGs related to photosynthesis were down-regulated in the susceptible M14 in comparison to the resistant WT. Moreover, the up-regulated WRKY transcription factors and down-regulated plant hormones related to plant growth were detected in the M14. The results suggest that down-regulated chloroplast genes, up-regulated WRKY transcription factors, and depressed plant hormones related to plant growth in the M14 might coordinately render the susceptibility though there was a significant high level of PRs. Those negative effectors might be triggered in the susceptible plant by fungal infection and resulted in reduction of photosynthesis and phytohormones and led to symptom formation.

MeSH terms

Arachis / genetics*
Gene Expression Profiling
Gene Expression Regulation, Plant*
Genotype
Mutation*
Photosynthesis
Plant Diseases / microbiology*
Plant Leaves / metabolism*
Sequence Analysis, RNA
Transcriptome

Grants and funding

This work was funded by grants to XYZ, National High-Tech R&D Program of China (863 program), 2013AA102602-6, http://program.most.gov.cn/; XYZ, China Agriculture Research System, CARS-13, http://119.253.58.231/; SYH, Science-Technology Foundation for Outstanding Young Scientists of Henan Academy of Agricultural Sciences, 2013YQ08, http://www.hnagri.org.cn/kygl/index.php. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.