Optimization of Surfactant-Mediated, Ultrasonic-assisted Extraction of Antioxidant Polyphenols from Rattan Tea (Ampelopsis grossedentata) Using Response Surface Methodology

Feng Li; Aun Raza; Yan-Wei Wang; Xiu-Quan Xu; Guan-Hua Chen

doi:10.4103/pm.pm_159_16

Optimization of Surfactant-Mediated, Ultrasonic-assisted Extraction of Antioxidant Polyphenols from Rattan Tea (Ampelopsis grossedentata) Using Response Surface Methodology

Pharmacogn Mag. 2017 Jul-Sep;13(51):446-453. doi: 10.4103/pm.pm_159_16. Epub 2017 Jul 19.

Authors

Feng Li^{1

2}, Aun Raza³, Yan-Wei Wang³, Xiu-Quan Xu³, Guan-Hua Chen¹

Affiliations

¹ Department of Food Science, School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212001, Jiangsu Province, P. R. China.
² Department of Cardiothoracic Surgery, Affiliated Hospital of Jiangsu University, Zhenjiang 212001, Jiangsu Province, P. R. China.
³ Department of Pharmacognosy, School of Pharmacy, Jiangsu University, Zhenjiang 212013, P. R. China.

Abstract

Background: Rattan tea is a medicinal plant that has been used for many years for the treatment of inflammation, fatty liver, tumor, diabetes, and hyperlipidemia.

Objective: A green and novel approach based on surfactant-mediated, ultrasonic-assisted extraction (SM-UAE) was developed for the extraction of antioxidant polyphenols from Rattan tea. A nonionic surfactant Tween-80 was selected as extraction solvent. The antioxidant activity was measured by total phenolic content (TPC) and ferric-reducing/antioxidant capacity (FRAC) assay.

Materials and methods: Optimization of extraction parameters including concentration of solvent, ultrasonic time, and temperature were investigated by response surface methodology. The antioxidant activity was measured by TPC and FRAC assay.

Results: The optimal extraction conditions were determined as 6.8% (v/v) of aqueous Tween-80, ultrasonic temperature of 54°C, and ultrasonic time of 28.8 min. Under these conditions, the highest TPC value of 360.4 mg gallic acid equivalent per gram of dry weight material (GAE/g DW) was recorded. Moreover, 6.8% (v/v) of aqueous Tween-80, ultrasonic temperature of 54.5°C, and ultrasonic time of 28.4 min were determined for the highest FRAC value of 478.2 μmol of Fe²⁺/g of weight material (μmol Fe²⁺/g DW). Compared with other methods, the TPC and FRAC values of 313.5 mg GAE/g DW and 389.6 μmol Fe²⁺/g DW were obtained by heat reflux extraction using ethanol as solvent, respectively, and 343.2 mg GAE/g DW and 450.1 μmol Fe²⁺/g DW were obtained by UAE using ethanol as solvent, respectively.

Conclusion: The application of SM-UAE markedly decreased extraction time or extraction cost and improved the extraction efficiency, compared with the other methods.

Summary: Surfactant-mediated ultrasonic-assisted extraction of antioxidant polyphenols from Rattan TeaResponse surface methodology used to optimize parameters and study combined effectsOptimized surfactant-mediated ultrasonic-assisted extraction process enhances the antioxidant phenolics extraction in less time. Abbreviations used: SM-UAE: Surfactant-mediated ultrasonic-assisted extraction; TPC: total phenolic content; FRAC: Ferric reducing antioxidant capacity; RSM: Response surface methodology; BBD: Box-Behnken design.

Keywords: Antioxidant polyphenols; Rattan tea; Tween-80 mediated extraction; response surface methodology; ultrasonic-assisted extraction.