Recent advances in signal amplification strategy based on oligonucleotide and nanomaterials for microRNA detection-a review

Biosens Bioelectron. 2018 Jan 15:99:612-624. doi: 10.1016/j.bios.2017.08.036. Epub 2017 Aug 18.

Abstract

MicroRNAs (MiRNAs) play multiple crucial regulating roles in cell which can regulate one third of protein-coding genes. MiRNAs participate in the developmental and physiological processes of human body, while their aberrant adjustment will be more likely to trigger diseases such as cancers, kidney disease, central nervous system diseases, cardiovascular diseases, diabetes, viral infections and so on. What's worse, for the detection of miRNAs, their small size, high sequence similarity, low abundance and difficult extraction from cells impose great challenges in the analysis. Hence, it's necessary to fabricate accurate and sensitive biosensing platform for miRNAs detection. Up to now, researchers have developed many signal-amplification strategies for miRNAs detection, including hybridization chain reaction, nuclease amplification, rolling circle amplification, catalyzed hairpin assembly amplification and nanomaterials based amplification. These methods are typical, feasible and frequently used. In this review, we retrospect recent advances in signal amplification strategies for detecting miRNAs and point out the pros and cons of them. Furthermore, further prospects and promising developments of the signal-amplification strategies for detecting miRNAs are proposed.

Keywords: MicroRNAs; Nanomaterials; Oligonucleotide; Review; Signal-amplification strategies.

Publication types

  • Review

MeSH terms

  • Biosensing Techniques / methods*
  • Biosensing Techniques / trends
  • Humans
  • MicroRNAs / genetics
  • MicroRNAs / isolation & purification*
  • Nanostructures / chemistry
  • Nucleic Acid Amplification Techniques / methods*
  • Nucleic Acid Amplification Techniques / trends
  • Oligonucleotides / chemistry
  • Oligonucleotides / genetics

Substances

  • MicroRNAs
  • Oligonucleotides