Pulmonary diseases such as fibrosis are characterized by structural abnormalities that lead to impairment of proper lung function. Stereological analysis of serial tissue sections allows detection and quantitation of subtle changes in lung architecture. Here, we describe a stereology-based method of assessing pathology-induced changes in lung structure.
Keywords: Design-based stereology; Intersection counting; Lung; Morphometry; Numerical density; Physical disector; Point counting; Surface density; Systematic uniform random sampling; Volume density.